mcherry galectin3 (Addgene inc)
Addgene inc is a verified supplier 
93
Structured Review
Addgene inc
mcherry galectin3
Mcherry Galectin3, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mcherry galectin3/product/Addgene inc
Average 93 stars, based on 22 article reviews
Mcherry Galectin3, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mcherry galectin3/product/Addgene inc
Average 93 stars, based on 22 article reviews
mcherry galectin3 - by Bioz Stars,
2026-04
93/100 stars
Images
1) Product Images from "In situ snapshots along a mammalian selective autophagy pathway"
Article Title: In situ snapshots along a mammalian selective autophagy pathway
Journal: Proceedings of the National Academy of Sciences of the United States of America
doi: 10.1073/pnas.2221712120
Figure Legend Snippet: Sequential slices back and forth through the representative tomogram in cross-section view and the 3D rendering models. The movie shows that the growing phagophore engulfing Salmonella establishes close interaction with the ER tube and the ER sheet at the two individual rim regions. The mCherry-GAL8 expressing HeLa cells were infected with GFP expressing Salmonellae and imaged at 1.5 hours p.i.. Related to Figure 2.
Techniques Used:
Figure Legend Snippet: Sequential slices back and forth through the representative tomogram in cross-section view and the 3D rendering models. The movie shows that Salmonella is encapsulated by the growing phagophore and the matured autophagosome. The mCherry-GAL8 expressing HeLa cells were infected with GFP expressing Salmonellae and imaged at 2 hours p.i.. Related to SI Appendix , Figure S2C.
Techniques Used:
Figure Legend Snippet: Enlarged sequential slices through the representative tomogram in cross-section view and the 3D rendering models. The movie shows membrane contact sites of a dilated phagophore rim with a ER tube and ER sheet bridged by macromolecule clusters. Scale bar represents 50 nm. The mCherry-GAL8 expressing HeLa cells were infected with GFP expressing Salmonellae and imaged at 1.5 hours p.i.. Related to Figure 4.
Techniques Used:
Figure Legend Snippet: Sequential slices back and forth through the representative tomogram in cross-section view and the 3D rendering models. The movie shows that omegasome-like structures are found in close proximity to the ER. The mCherry-DFCP1 expressing HeLa cells were infected with GFP expressing Salmonellae and imaged at 2 hours p.i.. Related to Figure 5.
Techniques Used:
Schindelin et al., 2012 ) and SIMcheck ( 
Figure 1 Line scans laterally (A and C) and axially (B and D) through single fluorescent beads at 525 nm (A and B) and 605 nm (C and D) emission in widefield and SIM images. Widefield data presented as circles and SIM as diamonds with Gaussians fits as lines. In (E–H) are parts of a raw SIM image of fluorescent mitochondria with decreasing stripe width. 2nd order stripes are 406, 361, 325 and 295 nm respectively. In (I) are line scans through Fourier transforms of the data in (E–H), with varying SIM illumination stripe widths, showing the peaks due to the first order stripes (around 1–1.8 μm −1 ) and second order (around 2.4 to 3.5 μm −1 ). As stripe width decreases, second order peaks decrease in amplitude and shift along the y axis. The black arrow indicates the stripe width, 396 nm, used for the experimental data in the paper. Representative brightfield image of vitrified U2OS cells (J), before (pre-exposure) and (K), after (post-exposure) seven cycles of cryoSIM imaging of green, red, and far red intracellular fluorophores (525 nm, 605 nm and 647 nm) showing that there is no localized ‘melting’ due to exposure to laser light for SIM purposes. In (L) and (M), are orthoslices of (J) and (K), respectively. SIM imaging from the last round of data collection shows the preserved cellular features: (N), green fluorescent lipid droplets, (O), red fluorescent endoplasmic reticulum and (P), far-red fluorescent mitochondria corresponding to the brightfield data shown in (K). " width="100%" height="100%">
Figure 2 (A) X-ray projection of a 16x16 μm FOV collected at the beamline B24 TXM using the 40 nm objective in a U2OS mammalian cell sample 1h after it has been exposed to infectious reovirus at MOI of 50. The zoomed view in square at the bottom left of the image is located outside the cell. The backing surface (Quantifoil™) can be identified by the regular pattern of circles (needed for blotting prior to vitrification). (B) The diagonal dotted line defines the line used to generate (B), a line profile of pixel intensity in Fiji ( 
Figure 6 (A–C) Representative cross-sections from three cells, (A), (B), and (C), in the control population of U2OS cells. Cellular structures are identified with arrows: pink for the gold nanoparticle fiducials (250 nm), purple for mitochondria, red for the nuclear envelope, orange for the endoplasmic reticulum, green for MVBs, light blue for endo/lysosomes, gray for nucleoli. (D) 2D X-ray mosaics of a grid area with reovirus mock-infected U2OS cells with the corresponding SIM fluorescence slice aligned. The areas where higher resolution X-ray data were collected are outlined in blue (two ROIs ) and expanded. (E) Areas of focus in single slices from the 3D tomograms are shown as four overlapping frames with (from left to right): X-ray absorption only, composite of X-ray absorption and red fluorescence, composite of X-ray absorption and green fluorescence and composite of X-ray absorption with both fluorescence signals. (F and G), same as (D) and (E) respectively but for a cell population 1 h after infection. (H and I), same as (D) and (E) respectively but for a cell population 2 h after infection. (J and K), same as (D) and (E) respectively but for a cell population 4 h after infection. Black bars are 1 μm and white bars 10 μm. In (E), spurious signals represent background levels of fluorescence in U2OS cells; both green and red signals are perfectly co-localized, weak (relative to those observed later in the infection) and are not associated with any distinct cytoplasmic structures. In (G), 1h after infection, a number of small but distinct vesicles are both Gal3 and MRV positive while in (I), smaller endosomes have now merged to give rise to MVBs that have distinct compartments infused with Gal3 while others have not been similarly compromised (presumably arising from concatenation of membranes from MRV-free vesicles). Carbon-rich virus-induced domed structures are also seen in single vesicles. In (K), fluorescence is shown in smaller vesicles alongside large vesicle superstructures shown in 
Pettersen et al., 2004 ). " width="100%" height="100%">
